EVALUATION OF GINGIVAL TISSUE SAMPLES FOR PREDICTING THE TIME OF DEATH USING HISTOLOGICAL AND BIOCHEMICAL TESTS

Authors

  • Oydinov Azizjon Ergashevich Author
  • Rajabov Shuxrat Yunusovich Author
  • Sultanova Norjon Davletovna Author
  • Xaldarov Davron Baxramovich Author

Abstract

Thanatochemistry, also known as the chemistry of death, is utilized to estimate the postmortem interval (PMI) and plays a crucial role in forensic investigations. The integration of biochemical analysis alongside traditional methods has gained significance, as it enables the detection of early postmortem changes in tissue specimens. This study aimed to establish a correlation between histological alterations and enzymatic activity in gingival tissue samples at various time intervals: immediately postmortem, 1 hour, 5 hours, 24 hours, and 48 hours after death.

References

1. C. Henssge, B. Madea. Estimation of the time since death in the early post-mortem period. Forensic Sci. Int., 144 (2–3) (2004), pp. 167-175.

2. A.V. Reddy, G. Sriram, T.R. Saraswathi, B. Sivapathasundharam. Histological assessment of cellular changes in gingival epithelium in ante-mortem and post-mortem specimens. J. Forensic Dent. Sci., 3 (2011), pp. 27-32.

3. R.H. da Silva, A. Sales Peres, R.N. de Oliveira, F.T. de Oliveira, S.H. Sales-Peres. Use of DNA technology in forensic dentistry. J. Appl. Oral. Sci., 15 (2007), pp. 156-161.

4. H. Salam, E. Shaat, M. Aziz, A. Moneim Sheta, H. Hussein. Estimation of postmortem interval using thanatochemistry and postmortem changes. Alex. J. Med., 48 (2012), pp. 335-344.

5. V. Poposka, A. Gutevska, A. Stankov, G. Pavlovski, Z. Jakovski, B. Janeska. Estimation of time since death by using algorithm in early postmortem period. Glob. J. Med. Res., 13 (2013), pp. 17-25.

6. W. Jetter. Post-mortem biochemical changes. J. Forensic Sci., 4 (1959), pp. 330-341.

7. Andrea E. Donaldson, I.L. Lamont. Biochemistry changes that occur after death: potential markers for determining post-mortem interval. Plos One, 11 (2013), pp. 1-10.

8. D.S. Pushparani. High acid phosphatase level in the gingival tissues of periodontitis subjects. J. Basic Clin. Pharm., 6 (2015), pp. 59-63.

9. Murray RK, Granner DK, Rodwell VW. Catabolism of proteins and of amino acid nitrogen. Harper’s Illustrated Biochemistry, 27 ed.: Lange Medical Books/McGraw-Hill.

10. S.J. Davies, D.C. Gaze, P.O. Collinson. Investigation of cardiac troponins in postmortem subjects: comparing ante-mortem and postmortem levels. Am. J. Forensic Med. Pathol., 26 (3) (2005), pp. 213-215.

11. Y.O. Poloz, D.H. O’Day. Determining time of death: temperature-dependent postmortem changes in calcineurin A, MARCKS, CaMKII, and protein phosphatase 2A in mouse. Int. J. Leg. Med., 123 (2009), pp. 305-314.

12. H. Mikami, K. Terazawa, T. Takatori, S. Tokudome, T. Tsukamoto, K. Haga. Estimation of time of death by quantification of melatonin in corpses. Int. J. Leg. Med., 107 (1) (1994), pp. 42-51.

13. F. Wehner, H.-D. Wehner, M.C. Schieffer, J. Subke. Delimitation of the time of death by immunohistochemical detection of insulin in pancreatic β-cells. Forensic Sci. Int., 105 (3) (1999), pp. 161-169.

14. F. Musshoff, H. Klotzbach, W. Block, F. Traeber, H. Schild, B. Madea. Comparison of postmortem metabolic changes in sheep brain tissue in isolated heads and whole animals using H-MR spectroscopy - preliminary results. Int. J. Leg. Med., 125 (2011), pp. 741-744.

15. K. Takimoto, T. Deguchi, M. Mori. Histochemical detection of acid and alkaline phosphatases in periodontal tissues after experimental tooth movement. J. Dent. Res., 47 (1968), p. 340.

16. D.S. Pushparani, S. Nirmala. Comparison of acid phosphatase and β-glucuronidase enzyme levels in type 2 diabetes mellitus with and without periodontitis. Int. J. Sci. Eng. Res., 4 (2013), pp. 1164-1168.

17. J. Payne, J.A. Busuttil, W. Smock. Forensic Medicine Clinical and Pathological Aspects. GMM Publishers, San Francisco, London (2003).

18. N. Gururaj, B. Sivapathasundharam. Post mortem findings in the gingiva assessed by histology and exfoliative cytology. J. Oral Maxillofac. Pathol., 8 (2004), pp. 18-21.

19. G.L. Pradeep, K. Uma, P. Sharada, N. Prakash. Histological assessment of cellular changes in gingival epithelium in ante-mortem and postmortem specimens. J. Forensic Dent. Sci., 1 (2009), pp. 61-65.

20. V. Mahalakshmi, N. Gururaj, R. Sathya, T.R. Sabarinath, B. Sivapathasundharam, S. Kalaiselvan. Assessment of histological changes in antemortem gingival tissues fixed at various time intervals: a method of estimation of postmortem interval. J. Forensic Dent. Sci., 8 (2016), pp. 114-121.

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Published

2025-02-28